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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: J Bone Miner Res. 2019 Oct 24;35(2):382–395. doi: 10.1002/jbmr.3882

Figure 5. SYK inhibitor GS-9973 restores alveolar bone volume in ligature-induced periodontitis.

Figure 5.

(A) Procedure of GS-9973 administration started one day before ligature placement. (B) Two-dimensional (2D) coronal plane μCT images through the middle of maxillary second molar (bottom) and three-dimensional (3D) μCT images surrounding the maxillary second molar (top). P: palatal side. B: buccal side. (C) Percentage (%) of alveolar bone loss against contralateral unligated side. (D) TRAP staining of alveolar bone between two buccal roots of the ligated second molar. 10-week-old male mice. Bar = 100 μm. (E) Histomorphometric analysis for TRAP-positive cells. p = 0.0195 (male) & 0.7118 (female) for N.Oc/BS. p = 0.0472 (male) & 0.9751 (female) for Oc.S/BS. (F) TRAP-positive osteoclasts with ruffled borders indicated by arrowheads. High magnification of the boxed area in (D). 10-week-old male mice. Bar = 10 μm. (G) Procedure of GS-9973 administration started two days after ligature placement. (H) 2D coronal plane μCT images through the middle of maxillary second molar (bottom) and 3D μCT images surrounding the maxillary second molar (top). P: palatal side. B: buccal side. (I) Percentage (%) of alveolar bone loss against contralateral unligated side. (J) TRAP staining of alveolar bone between two buccal roots of the ligated second molar. 10-week-old male mice. Bar = 100 μm. (K) Histomorphometric analysis for TRAP-positive cells. p = 0.0216 (male) & 0.9469 (female) for N.Oc/BS. p = 0.0185 (male) & 0.5551 (female) for Oc.S/BS. (L) TRAP-positive osteoclasts with ruffled borders indicated by arrowheads. High magnification of the boxed area in (J). 10-week-old male mice. Bar = 10 μm. Data are presented as mean ± SD. *p < 0.05. NS = not significant. Student’s t-test.