A) Phosphorus balance by age (20- or 30-weeks) and disease (CKD or NL). There was a main effect for age where 20-week-old rats had a more positive phosphorus balance compared to both 30-week-olds, and CKD rats tended to have a more negative balance compared to NL, with no significant age x disease interaction. B) Calcium balance by age and disease. There was a main effect for age where 20-week-old rats had a more positive calcium balance compared to 30-week-olds, but there was no significant effect of disease and no significant age x disease interaction. Balance for each mineral was calculated as intake − fecal + urine. Means and standard error bars are shown for each group. The CKD group is shown in black bars and white circles, and NL white bars with black circles. ANOVA p-values for the overall model (PModel), main effect of age (PAge), main effect of disease (PDisease), and interaction of age and disease (PAxD) are shown. n=49 NL and n=47 CKD for both phosphorus and calcium, * p < 0.05, ** p < 0.01, *** p < 0.0001.