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. Author manuscript; available in PMC: 2021 Feb 1.
Published in final edited form as: J Inorg Biochem. 2019 Nov 13;203:110922. doi: 10.1016/j.jinorgbio.2019.110922

Table 5.

The Change in pHe over 48 h in Various Cell Lines and Uptake of Complex 3.

Cell Line Δ(pHe) over 48 ha MFIb IC50 Dark for 3c IC50 Light for 3c
MCF10A −0.32 ± 0.01 1189 ± 654 58 29
MDA-MD-231 −0.49 ± 0.09 181 ± 84 190 3.7
MCF7 −0.12 ± 0.03 596 ± 206 490 4.1
HeLa −0.88 ± 0.07 322 ± 134 730 120
a

The external pH was monitored for 48 h without changing the media. Each result is the average of five experiments.

b

Mean fluorescence intensity (MFI) is assumed to be proportional to uptake of 3. See the experimental section for a discussion of the limitations of this assumption. All cell lines were treated with 50 μM of 3 in the dark. These experiments were under typical conditions which included changing the media regularly.

c

IC50 values were reported previously and are shown in μM.[14]