TABLE 2.
Primer information.
| Primer | Sequence (5′–3′)a | Plasmids |
| T7 | TAATACGACTCACTATAGGG | pET22b(+) |
| T7T | GCTAGTTATTGCTCAGCGG | |
| pTRC99C-F | TTGCGCCGACATCATAAC | pTrc200HA |
| pTRC99C-R | CTGCGTTCTGATTTAATCTG | |
| rpsL-fw | AAAAACTCGAGGCAAAAGCTAAAACCAGGA | (1) pRL-rpsL |
| rpsL-rv | AAAAATCTAGACTTACTTAACGGAGAACCA | (2) pRL-rpsLStr |
| galK-fw | AAAAACTCGAGCAGTCAGCGATATCCATT | pRL-galk |
| galK-rv | AAAAATCTAGAGCAAAGTTAACAGTCGGT | |
| nupG-fw | AAAAACTCGAGTCAAACACTCATCCGCAT | pRL-nupG |
| nupG-rv | AAAAATCTAGACCCGTTTTTCTTTGCGTAA | |
| NptII-fw-XhoI | AAAAACTCGAGAGACTGGGCGGTTTTATGGA | pNptII |
| NptII-rv-HindIII | AAAAAAAGCTTCTCTAGCGAACCCCAGAGTC | |
| ClpP-SacI-fw | AAAAAGAGCTCATGTCATACAGCGGCGAACGAGATAAC | pClpP-HA |
| ClpP-PstI-rv | AAAAACTGCAGATTACGATGGGTCAGAATCGAATCGAC | |
| ClpA-SacI-fw | AAAAAGAGCTCATGCTCAATCAAGAACTGGAACTCAGTTT | pClpA-HA |
| ClpA-PstI-rv | AAAAACTGCAGATGCGCTGCTTCCGCCTTGTGCTTT | |
| ClpP-S111A-fw | TGTATGGGCCAGGCGGCCGCGATGGGCGCTTTCTTGCTG | (1) pClpPS111A-HA |
| ClpP-S111A-rv | CAGCAAGAAAGCGCCCATCGCGGCCGCCTGGCCCATACA | (2) pClpPS111A-tev-His |
| ClpP-H136A-fw | AATTCGCGCGTGATGATTGCCCAACCGTTGGGCGGCTAC | (1) pClpPH136A-HA |
| ClpP-H136A-rv | GTAGCCGCCCAACGGTTGGGCAATCATCACGCGCGAATT | (2) pClpPH136A-tev-His |
| ClpP-D185A-fw | GAACGTGATACCGAGCGCGCTCGCTTCCTTTCCGCCCCT | (1) pClpPD185A-HA |
| ClpP-D185A-rv | AGGGGCGGAAAGGAAGCGAGCGCGCTCGGTATCACGTTC | (2) pClpPD185A-tev-His |
| ClpP-R26A-fw | GTCATTGAACAGACCTCAGCCGGTGAGCGCTCTTTTGAT | pClpPR26A-HA |
| ClpP-R26A-rv | ATCAAAAGAGCGCTCACCGGCTGAGGTCTGTTCAATGAC | |
| ClpP-D32A-fw | CGCGGTGAGCGCTCTTTTGCTATCTATTCTCGTCTACTT | pClpPD32A-HA |
| ClpP-D32A-rv | AAGTAGACGAGAATAGATAGCAAAAGAGCGCTCACCGCG |
aRestriction enzyme sites are underlined, and mutated sequences are indicated in bold type.