Table 1.
Key resources.
| Reagent or resource* | Source | Identifier |
|---|---|---|
| Antibodies | ||
| S100A6 | R&D | AF4584 |
| LCP1 | Cell Signaling | 3588 |
| NOTCH1 | Cell Signaling | 3608 |
| Acetylated tubulin (Tuba4a) | Sigma-Aldrich | T6793 |
| S100A1 | NeoMarkers | RB-044-A0 |
| DSTN | Sigma | D8815 |
| Calbindin | Santa Cruz | Sc-365360 |
| RNAScope in situ probes | ||
| Mm-S100a6 | Advanced Cell Diagnostics | 412981 |
| Mm-Lcp1 | Advanced Cell Diagnostics | 487751 |
| Mm-Pirb | Advanced Cell Diagnostics | 496031 |
| Mm-Slc2a3 | Advanced Cell Diagnostics | 438851 |
| Mm-Spry2 | Advanced Cell Diagnostics | 425061 |
| Mm-Birc5 | Advanced Cell Diagnostics | 422701 |
| Mm-Notch2 | Advanced Cell Diagnostics | 425161 |
| Hs-TUBA1B | Advanced Cell Diagnostics | 529451 |
| Mm-Myh9 | Advanced Cell Diagnostics | 556881 |
| Mm-Nlrp3 | Advanced Cell Diagnostics | 439571 |
| Mm-Cdkn1b | Advanced Cell Diagnostics | 499991 |
| Mm-Pla2g7 | Advanced Cell Diagnostics | 453811 |
| Mm-Ppib | Advanced Cell Diagnostics | 313911 |
| Dap8 | Advanced Cell Diagnostics | 310043 |
| Reagents and Kits Critical for Immunohistochemistry and in situ hybridization | ||
| SCEM (embedding medium) | http://section-lab.jp/index.html | SCEM |
| Cryofilm type 2C (Adhesive film) | http://section-lab.jp/index.html | Cryofilm type 2C |
| Critical Commercial Assays | ||
| mRNA-Seq on C1 | ||
| Nextera XTIndex Kit V2 setB | Illumina | 15052164 |
| TRuSeq Dual Index Sequencing Primers-Paired End | Illumina | 15029399 |
| Nextera XT Sample Prep Kit | Illumina | 15032354 |
| C1 Single-Cell Auto Prep Module2 | Fluidigm | 100–5519 |
| Module2 (mRNA Seq) | Fluidigm | 100–6209 |
| Quant-iT PicoGreen dsDNA Assay Kit | Molecular Probes | P11496 |
| Advantage 2 PCR kit | Takara-Clontech | 639207 |
| SMART-Seq v4 Ultra Low Input RNA Kit for the Fluidigm C1 System | Takara-Clontech | 635028 |
| SMARTer Ultra Low RNA Kit for the Fluidigm C1 System | Takara-Clontech | 634835 |
| DynaMag—PCR | Invitrogen | 49–2025 |
| Agencourt AMPure XP | Beckman-Coulter | A63880 |
| LIVE/DEAD Viability/Cytotoxicity Kit | Invitrogen | L3224 |
| Cell strainer, 40 μm | Falcon | 352340 |
| qPCR on C1 | ||
| SsoFast EvaGreen Supermix with low ROX | Bio-Rad | 172–5211 |
| DNA Suspension Buffer | Teknova | T0221 |
| Single Cell-to-CT Kit | Invitrogen | 4458237 |
| GE 96.96 Dynamic Array DNA Binding Dye Loading Reagent Kit | Fluidigm | 100–3415-R |
| ddPCR on QX200TM AutoDGTM Droplet DigitalTM PCR System | ||
| ddPCRTM 96-Well PCR Plates | Bio-Rad | 12001925 |
| DG32TM Cartridges | Bio-Rad | 1864108 |
| PCR PlateHeat Seal, foil, pierceable | Bio-Rad | 1814040 |
| Automated Droplet Generation Oil for EvaGreen | Bio-Rad | 1864112 |
| ddPCRTM Droplet Reader Oil | Bio-Rad | 1863004 |
| Deposited Data | ||
| FACS-purified adult cochlear supporting cell single-cell RNA-Seq (Fluidigm C1) | This article | |
| Experimental Models: Organisms/Strains | ||
| Tg(Lfng-EGFP)HM340Gsat BAC transgenic mouse line (LfngEGFP) | GENSAT (Gong et al., 2003) | |
| Software and Algorithms | ||
| Seurat v2.0 | https://satijalab.org/seurat/ | |
| SINGuLAR v3.6.2 | https://www.fluidigm.com/software |
*Contact for Reagent and Resource Sharing.