Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 11;3:62. doi: 10.1038/s42003-020-0796-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a Schematic of ASFV detection, which combines CRISPR/Cas12a and lateral flow detection. The ssDNA reporter was labelled with digoxin and biotin (ssDNA-DB-reporter) at the 5’ and 3’ termini, respectively. The immunochromatographic strip using Au-NP anti-digoxin antibody to show the readout. The sample band was only shown when the ssDNA-DB-reporter was cleaved by CRISPR/Cas12a, which is activated by ASFV DNA. b ASFV detection with CRISPR/Cas12a-LFD at 37 ^oC in 30 min. The top band is the test band, and the bottom band is the control band. No colour change at the test line was observed for the two tested swine DNA viruses, PRV and PCV2. c The band intensity of lateral flow strip in (b) were further quantified by ImageJ and visualization with GraphPad. d The limit of detection of CRISPR/Cas12a-LFD combined with RAA. Serially diluted synthetic ASFV DNA was used as a template. e The visualization of sample bands intensity were quantified by ImageJ based on data in (d).