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. 2020 Feb 11;3:62. doi: 10.1038/s42003-020-0796-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Schematic diagram of ASFV DNA detection in clinical blood samples using CRISPR/Cas12a-LFD without DNA extraction. To detect ASFV DNA in an hour, the clinical sample DNA was released in 3 min, followed by 20 min for RAA replication and 30 min for the CRISPR/Cas12a reaction; then, readout with lateral flow detection occurred in 3 min. b The detection of ASFV in serum samples with CRISPR/Cas12a-LFD. The DNA from clinical serum samples was released and preamplified. The CRISPR/Cas12a reaction and readout in lateral flow strips were then performed. The control band is shown at the bottom, and the test band is shown at the top. c Quantitation of sample bands in (b). The sample bands were scanned and analysed with ImageJ software. d The correlation analysis of ASFV detection results generated with CRISPR/Cas12a-LFD and real-time PCR. The same sample detection Ct value of real-time PCR and band intensity value of the lateral flow strip were co-analysed. The correlation value was analysed with GraphPad. e The Venn diagram shows the consistency between the Cas12a-LFD and real-time PCR assays.