Fig. 2. Structure of EF-bound PA7 channel (PA7-EF).
a Overall structure of PA7-EF shown as ribbon. b Zoom-in view (view 1) of the PA7 α-clamp site showing its detailed interactions with α1 of EF. The cryo-EM density is shown as semi-transparent gray. c Rotated view 1 showing structure comparison of the substrate-binding α-clamp between PA7-EF channel (color) and PA8-(LFN)4 pre-channel (gray), except that the density is not shown for clarity. d Zoom-in view (view 2) showing the details of the PABD domain of EF binding to PAN and PAC. e Rotated view 2 showing the superposition of PA-bound EF (purple for EF, orange for PA) and PA-bound LF (gray for both PA and LF), except that the density is not shown for clarity. Hydrogen bonds are shown as dashed lines.