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. 2020 Feb 11;10:2345. doi: 10.1038/s41598-020-59410-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DNA methylation analysis of oocytes derived from early-postpartum (Epp), late postpartum (Lpp), and cyclic heifers. (a) Venn diagram of DMRs obtained by logistic regression (10% methylation difference, adjusted p < 0.05), with number of hyper- and hypo-methylated DMRs in each comparison, where 405 DMRs were found to be shared across all conditions (center). (b) Top 20 differentially methylated regions overlapping gene (GSy = gene symbol) body regions and their methylation changes in all three comparisons. (c) Fold methylation changes found in hyper- (right) and hypo-methylated (left) DMRs from pairwise comparisons.