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. 2020 Feb 11;11:836. doi: 10.1038/s41467-020-14545-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Only non-essential genes whose knockout does not prevent cell division are deletion candidates and are equally divided into Sets A–D. Four hundred random deletion subsets are produced and simulated per set, each containing 50–100% of the genes within the set. Deletion subsets that do not prevent division (“viable”) go to the next stage. b 3000 combinations of deletion subsets are generated and simulated. c This is a cyclical step. The mutation pool targets a random number of genes for alteration (both knock-ins and knockouts), including essential genes. Simulation data generated by the GAMA algorithm is available³⁷ (see Data availability section). Details of simulations settings are available in the Methods section.