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. 2020 Feb 11;10:2337. doi: 10.1038/s41598-020-58120-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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KCC2 expression is low in progenitor-derived neurons by 7 DIV. (a) Little KCC2 immunofluorescence was observed in progenitor-derived neurons and P5 RGCs compared with P14 RGCs. Scale in (a) is 50 µm. (b) Faint protein bands corresponding to KCC2 monomer and dimer were observed in progenitor derived neurons (7 DIV) and early postnatal retinas (P5) while retinal KCC2 was greatly upregulated by postnatal day 14 (P14). 7 DIV and P5/P14 blots were run separately.