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. 2020 Feb 11;10:2337. doi: 10.1038/s41598-020-58120-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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KCC2 is upregulated in progenitor-derived neurons by 21 DIV. (a) Example images of KCC2 immunofluorescence in retinal progenitor-derived neurons at 21 DIV in all three insert conditions as marked. Scale bar is 50 µm. (b) Immunofluorescence measurements of relative KCC2 protein levels in all 3 conditions at 7 DIV and 21 DIV. (c) qPCR data showed >150-fold increase in KCC2 mRNA expression in progenitor-derived neurons from 7 DIV to 21 DIV in all three insert conditions, but no difference among the three insert conditions. (d) qPCR data show no significant differences in GABA_A receptor subunit expression between 7 DIV and 21 DIV or among the three insert conditions. In (b–d) red lines indicate means; error bars, S.D.s; n.s. difference not significant; *p < 0.05. Immunofluorescence and qPCR data were normalized to values at 7 DIV with no insert.