Table 3.
Performance comparison of the previously reported using SPR-based DENV sensor.
| SPR substrate | Surface modification | Target | Sensitivity, S and dissociation constant, KD | Limit of detection |
|---|---|---|---|---|
| Gold49 | Carboxymethylated dextran/N-ethyl-N-(dimethylaminopropyl) carbodiimide-N-hydroxysuccinimide (EDC-NHS) | IgM | KD = 5 nM | 1 nM |
| Gold29 | Carboxymethylated dextran/EDC-NHS | DENV-2 NS1 | KD = 12.3 nM | 0.25 ng/mL |
| Gold50 | 16-mercaptohexadecanioc acid/EDC-NHS | IgM | — | 12 pg/mm2 |
| Gold51 | 16-mercaptohexadecanioc acid/EDC-NHS | NS1 | — | 5.73 pg/mm2 |
| Gold52 | Carboxymethylated dextran/EDC-NHS | IgM |
S = 0.0132 pM/sec−1 KD = 2.12−12 nM |
2.125 pM |
| Gold (our work) | Dithiobis (succinimidyl undecanoate)/reduced graphene oxide-polyamidoamine dendrimer/EDC-NHS | DENV-2 E-proteins |
S = 0.25762 °/pM KD = 0.1496 pM |
0.08 pM |