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. 2020 Feb 11;10:2320. doi: 10.1038/s41598-020-59146-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Visualisation of FA in U87 cells migrating across microchannels. (A) The amount of Tf@pSiNP, BSA@pSiNP, and Tf associated with U87 cells which migrated across microchannels as indicated by Cy5 intensity normalised to their respective non-migrated cells. Higher value indicates higher cell-pSiNP or cell-Tf associations in migrated cells. (B) Immunofluorescence staining of vinculin as a representation of FA. Filament actin, red. Nucleus, blue. Vinculin, green and white is Cy5-labelled Tf@pSiNP, BSA@pSiNP, and Tf. (C) Aspect ratio of identifiable FA in U87 cells. (n = 5, error bar = ±1 SD. * indicates p < 0.05).