Figure 6.

Time-lapse imaging of FA dynamics in U87 cells migrating across microchannels. (A) Stages of untreated cell migration across the microchannels. Cell (a) migrated to the entrance of microchannels; Leading front of cell (b) was protruding across the microchannels, while a tail was retracting (arrows); Cell (c) was at the intermediate stage where cell body spanned across entire microchannel. Cytoplasmic mCherry, red. Talin-GFP, green. (B) Talin-GFP expressing U87 cells treated by Tf@pSiNP, BSA@pSiNP, or left untreated, at their intermediate stages of migration across the microchannels. White arrows indicate observable FA at the leading fronts of the cells.