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. 2020 Feb 11;3:60. doi: 10.1038/s42003-020-0784-9

Fig. 2. The Eleanor2 RNA destabilizes the nucleosome.

Fig. 2

a Schematic representation of the nucleosome thermal stability assay. In this assay, SYPRO Orange binds to the hydrophobic surfaces of heat-denatured histones, but not DNA and RNA. The fluorescence signal from SYPRO Orange bound to histones is detected. The first and second peaks correspond to the dissociation phases of the H2A–H2B and H3–H4 complexes from the nucleosome, respectively. b Thermal stability curves of the nucleosome (1.25 μM) in the presence or absence of Eleanor2 DNA (0.62 μM) or RNA (1.25 μM). The fluorescence intensity was plotted against the temperature (from 30 °C to 95 °C). The means ± s.d. (n = 3) are shown. c Thermal stability curves of the H3–H4 and DNA complex (1.25 μM) in the presence or absence of the Eleanor2 RNA (1.25 μM). The means ± s.d. (n = 3) are shown. d Thermal stability curves of the nucleosome (1.25 μM) with increasing amounts of the Eleanor2 RNA (0.125, 0.625, 1.25, and 2.5 μM). Means ± s.d. (n = 3) are shown. The source data for the thermal stability assay are shown in Supplementary Fig. 6.