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. 2020 Feb 11;3:60. doi: 10.1038/s42003-020-0784-9

Fig. 3. Thermal stability assay with the Eleanor2 RNA fragments.

Fig. 3

a The Eleanor2 RNA secondary structures predicted by CentroidFold. RNA fragments employed in this assay are encircled by red ellipses with base numbers, 250–302 and 320–447. b Purified Eleanor2 RNA fragments transcribed in vitro were analyzed by 8% polyacrylamide/7 M urea denaturing gel electrophoresis with ethidium bromide staining. The uncropped gel image is shown in Supplementary Fig. 5. c Thermal stability curves of the nucleosome (1.25 μM) with Eleanor2 RNA fragments. Equimolar amounts (in moles of nucleotides) of the full-length Eleanor2 RNA (1–656) (1.25 μM), Eleanor2 (250–302) (14.0 μM), and Eleanor2 (320–447) (6.17 μM) were added to each reaction solution. The fluorescence intensity was plotted against the temperature (from 30 °C to 95 °C). Means ± s.d. (n = 3) are shown. The source data for the thermal stability assay are shown in Supplementary Fig. 7.