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. 2020 Feb 11;3:60. doi: 10.1038/s42003-020-0784-9

Fig. 4. Thermal stability assay with other ncRNAs.

Fig. 4

a Thermal stability curves of the nucleosome (1.25 μM) in the presence of MALAT1 RNA (0.22 μM). The amount of MALAT1 RNA (0.22 μM) corresponds to 1.25 μM Eleanor2 RNA in weight. Means ± s.d. (n = 3) are shown. b Thermal stability curves of the nucleosome (1.25 μM) in the presence of DSCAM-AS1 RNA (0.33 μM). The amount of DSCAM-AS1 RNA (0.33 μM) corresponds to 1.25 μM Eleanor2 RNA in weight. Means ± s.d. (n = 3) are shown. c Thermal stability curves of the nucleosome (1.25 μM) in the presence of XIST RNA (0.27 μM). The amount of XIST RNA (0.27 μM) corresponds to 1.25 μM Eleanor2 RNA in weight. Means ± s.d. (n = 3) are shown. d Thermal stability curves of the nucleosome (1.25 μM) in the presence of ESR1 mRNA (0.46 μM). The amount of ESR1 mRNA (0.46 μM) corresponds to 1.25 μM Eleanor2 RNA in weight. Means ± s.d. (n = 3) are shown. e Thermal stability curves of the nucleosome (1.25 μM) in the presence of poly(U) (265 ng/μl). The amount of poly(U) (265 ng/μl) corresponds to 1.25 μM Eleanor2 RNA in weight. Means ± s.d. (n = 3) are shown. The source data for the thermal stability assay are shown in Supplementary Fig. 8.