Figure 1.

Regulatory associated protein with mammalian/mechanistic target of rapamycin (RAPTOR) is overexpressed in colorectal cancer (CRC) tissues and is positively associated with URB1 expression, poor clinicopathological features, and prognosis of patients with CRC. A and B, Expression of RAPTOR and URB1 in human colon cancer tissues based on The Cancer Genome Atlas (TCGA) datasets (http://ualcan.path.uab.edu). C, RAPTOR mRNA expression level was measured in paired clinical CRC samples by using quantitative real‐time PCR. D and E, RAPTOR protein expression level was assessed in clinical CRC tissue microarray (TMA) through immunohistochemistry (IHC) staining. Scale bars: 50 μm (×400 magnification). F, Overexpression of RAPTOR was associated with a greater T stage and TNM stage in patients with CRC. G, URB1 protein expression level was assessed in TMA through IHC staining. Scale bars: 50 μm (×400 magnification). H and I, RAPTOR exhibited a synchronized expression pattern and was positively correlated with URB1 by utilizing IHC analysis. Scale bars: 100 μm (×40 magnification). Scale bars: 50 μm (×400 magnification). J and K, Patients with CRC had a high expression of RAPTOR and showed inferior overall survival as compared to that of patients with a low expression of RAPTOR based on this study and Gene Expression Omnibus data. Data are mean ± SD, ns, not significant, *P < .05, **P < .01, ***P < .001 and ****P < .0001