Figure 4.

Regulatory associated protein with mammalian/mechanistic target of rapamycin (RAPTOR) silencing or rapamycin treatment inactivates mTOR complex 1 (mTORC1) and downregulates URB1 and cyclinA2 (CCNA2) expression. A and B, The mRNA and protein expression levels of mTORC1 components and substrates. The mRNA and protein levels of URB1 and CCNA2 in RAPTOR silencing RKO cells were measured via quantitative real‐time PCR and western blot, respectively. C, Western blot was used to assess the inhibitory effect of rapamycin at different concentrations on URB1 and CCNA2 expression and mTORC1 activity in CRC cells. Rapamycin concentrations used in this study included 50 and 100 nmol/L. Data are mean ± SD (n = 3), *P < .05, **P < .01 and ***P < 0.001