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. 2020 Feb 5;11:122. doi: 10.3389/fmicb.2020.00122

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Copyright © 2020 Meng, Huang, Huang, Liu, Han and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transcriptional changes in the mutant strains of Y. enterocolitica. Y. enterocolitica grown to the mid-log phase in LNNS medium and total RNA was extracted. Expression of opgGH, flhDC, ftsAZ, hmsHFRS, and hmsT were determined by RT-qPCR in vitro. The 16S rRNA gene was used as a normalization control. (A) Gene transcription in ΔopgGH and ΔopgGH-P_BADopgGH strains. (B) Gene transcription in ΔrcsF, ΔrcsC, ΔrcsB, ΔopgGH-ΔrcsF, ΔopgGH-ΔrcsC, and ΔopgGH-ΔrcsB mutant strains. (C) Gene transcription in ΔenvZ, ΔompR, ΔopgGH-ΔenvZ, and ΔopgGH-ΔompR mutant strains. (D) Gene transcription in ΔopgGH-ΔrcsF-P_BADopgGH, ΔopgGH-ΔrcsC-P_BADopgGH, ΔopgGH-ΔrcsB-P_BADopgGH, ΔopgGH-ΔenvZ-P_BADopgGH, and ΔopgGH-ΔompR-P_BADopgGH strains. The data are presented as the mean ± SD of at least three independent experiments.