TABLE 1.
Strains and plasmids used in this study.
| Strains and plasmids | Relevant characteristics | Sources |
| Y. enterocolitica | ||
| ATCC23715 | WT, serotype O:8, Biotype 1B, pYV– | Lab stock |
| YE-1 | △opgGH | This study |
| YE-2 | △opgGH, PBADopgGH; Ampr | This study |
| YE-B | △rcsB | Meng et al., 2019 |
| YE-C | △rcsC | This study |
| YE-F | △rcsF | This study |
| YE-1B | △opgGH, △rcsB | This study |
| YE-1C | △opgGH, △rcsC | This study |
| YE-1F | △opgGH, △rcsF | This study |
| YE-2B | △opgGH, △rcsB, PBADopgGH; Ampr | This study |
| YE-2C | △opgGH, △rcsC, PBADopgGH; Ampr | This study |
| YE-2F | △opgGH, △rcsF, PBADopgGH; Ampr | This study |
| YE-Z | △envZ | This study |
| YE-R | △ompR | Meng et al., 2019 |
| YE-1Z | △opgGH, △envZ | This study |
| YE-1R | △opgGH, △ompR | This study |
| YE-2Z | △opgGH, △envZ, PBADopgGH; Ampr | This study |
| YE-2R | △opgGH, △ompR, PBADopgGH; Ampr | This study |
| E. coli | ||
| S17-1 λpir | recA1, thi, pro, hsdR-M +, RP4:2-Tc:Mu–Kan:Tn7, λpir | Lab stock |
| DH5a | F–, φ80lacZ△M15, △(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17 (rk–,mk+), phoA, supE44, λ–, thi-1, gyrA96, relA1 | Lab stock |
| Plasmids | ||
| pDS132 | Conditional replication vector; | Lab stock |
| R6K origin, mobRK4 transfer origin, sucrose-inducible-sacB; Cmr | ||
| pDS132-△opgGH | Upstream and downstream opgGH fragments | This study |
| were cloned into pDS132; Cmr | ||
| pDS132-△rcsB | Upstream and downstream rcsB fragments | This study |
| were cloned into pDS132; Cmr | ||
| pDS132-△rcsC | Upstream and downstream rcsC fragments | This study |
| were cloned into pDS132; Cmr | ||
| pDS132-△rcsF | Upstream and downstream rcsF fragments | This study |
| were cloned into pDS132; Cmr | ||
| pDS132-△envZ | Upstream and downstream envZ fragments | This study |
| were cloned into pDS132; Cmr | ||
| pDS132-△ompR | Upstream and downstream ompR fragments | This study |
| were cloned into pDS132; Cmr | ||
| pBAD24 | AraC, promoter PBAD; Ampr | Lab stock |
| pBAD24-opgGH | AraC, PBAD opgGH; Ampr | This study |
Amp, ampicillin; Cm, chloramphenicol; r, resistance.