Figure 7.

AntimiR-145 Upregulates Sets of Interferon-Regulated Genes

Gene set enrichment analysis (GSEA) was conducted to demonstrate differential gene expression in HDM-sensitized mice and HDM-sensitized mice treated with antimiR-145. HDM-sensitized mice served as the reference group for analysis of poly(A) RNA-seq data. A ranked set of differentially expressed genes (FDR < 0.2) was generated using DESeq2, and GSEA was performed using sets of interferon-regulated genes included in the Hallmark dataset of the MSigDB collection. (A) AntimiR-145 (black symbols) and nontargeting oligonucleotide (blue symbols) both significantly increased expression of most interferon-alpha-regulated genes (p < 0.001). (B) Treatments with antimiR-145 and nontargeting oligonucleotide also significantly enhanced interferon-beta-regulated genes (p < 0.001; n = 10 for each treatment).