Figure 8.

Oligonucleotide Treatment Reduces Immune Cell Infiltration into BALF

(A–D) Differential counts of immune cells in BAL fluid (BALF) were performed by flow cytometry: (A) total CD45+ cells, (B) eosinophils, (C) neutrophils, and (D) alveolar macrophages. Statistical hypothesis testing was performed by Kruskal-Wallis ANOVA and Dunn’s post hoc test. *p < 0.05; ***p < 0.005; ****p < 0.001. n = 10–18 mice. (E) Oligonucleotide treatment reduces tissue inflammation. Sections of FFPE lung were stained with H&E to identify sites of inflammation (purple clusters). A representative micrograph for each treatment group is shown. (F) Volume density of inflamed sites was quantified by measuring the area of each site divided by the total cross-sectional area of each lobe. Statistical hypothesis testing was performed with Kruskal-Wallis ANOVA and Dunn’s post hoc test using HDM-sensitized mice as the reference group. ***p < 0.005. n = 10–17.