Fig. 1.

Quantitative analysis of relative overexpression levels and cellular localization of SCD1 and SREBF1 in engineered cells. Figure A shows the relative abundance of SCD1 as calculated using densitometry from the western blots reported in Fig. 2A and B of [1], whilst figure B shows the relative abundance of SREBF1 species. The values have been normalized to L7α loading control and subsequently normalized to either CHO-SCD1^POOL (A) or CHO-SREBF1^POOL (B) values. Cellular localization of overexpressed SCD1 and SREBF1 proteins in CHO-Control^POOL, CHO-SCD1^POOL and CHO-SREBF1^POOL cell pools as determined by immunofluorescent detection using an anti-V5 antibody conjugated with a FITC secondary antibody (C). An anti-calnexin antibody conjugated with a TRITC secondary antibody was used to highlight the position of the ER. Images were obtained using confocal microscopy.