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. 2020 Jan 31;29:105217. doi: 10.1016/j.dib.2020.105217

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© 2020 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Product analysis post transient transfection in lipid modified cells. Previously engineered host cells (CHO-Control^POOL, CHO-SCD1^POOL, CHO-SREBF1^POOL) were transiently transfected with vectors containing genes for expression of either cB72.3 (A and B) or FcFP (C and D). Quantitative analysis using an Octet® was carried out on supernatant samples harvested at 24, 48, 72 and 96 h post transfection for cB72.3 (A) and FcFP (C) transfections. Western blot analysis of cB72.3 (B) and FcFP (D) molecules were carried out on supernatant samples harvested at 96 h, and 48 h in the case of cB72.3. n = 3 for each data point and error bars show ± one standard deviation.