Fig. 4.

Product analysis post transient transfection with vectors bearing genes for a recombinant product and a lipid modifying gene on the same expression vector. Vectors containing genes necessary for expression of an antibody fusion molecule (IL2-F) with either no additional LMM gene (IL2-F-Control), the SCD1 gene (IL2-F-SCD1) or the SREBF1 gene (IL2-F-SREBF1) on the same vector. Quantitative analysis using an Octet® was carried out on supernatant samples harvested at 96 h post transfection to measure relative product concentration (A). Western blot analysis was also carried out on reduced samples. A heavy chain band (approximately 50 kDa) and an antibody heavy chain-IL2 fusion band (approximately 70 kDa) associated with the antibody fusion molecule is observed (B). n = 3 for each data point and error bars show ± one standard deviation.