Table 2.
Details of antibodies used for analysis including the manufacturer and reference, the organism produced in and its applications reported in this article and Budge et al. [1], and the dilution used.
| Antibody | Company/Catalogue Number | Produced In | Use and Dilution |
|---|---|---|---|
| Primary Antibodies | |||
| Anti-V5 | Sigma/V8012 | Mouse | Immunofluorescence- 1:500 |
| Anti-calnexin | Abcam/ab22595 | Rabbit | Immunofluorescence- 1:200 |
| Anti-γ chain | Sigma/I9764 | Rabbit | Western Blot- 1:2000 |
| Anti-SCD1 | Cell Signalling/2438 | Rabbit | Western Blot- 1:1000 |
| Anti-SREBP1 | Abcam/ab3259 | Mouse | Western Blot- 1:1000 |
| Anti-L7α | Kind gift from Dr. Anne Roobol | Rabbit | Western Blot (used at supplied concentration) |
| Secondary Antibodies | |||
| Anti-Mouse FITC conjugate | Sigma/F0257 | Goat | Immunofluorescence 1:250 |
| Anti-Rabbit FITC conjugate | Sigma/F9887 | Goat | Immunofluorescence 1:250 |
| Anti-Mouse HRP Peroxidase conjugate | Sigma/A4416 | Goat | Western Blot- 1:2000 |
| Anti-Rabbit HRP Peroxidase conjugate | Sigma/A6154 | Goat | Western Blot- 1:2000 |