Specifications Table

Subject	Biotechnology
Specific subject area	Lipid Metabolism Metabolic Engineering and Recombinant Protein Expression from CHO Cells
Type of data	Table Image Chart Graph Figure
How data were acquired	Viable cell concentration and culture viability measurements were collected on a ViCell instrument (Beckman Coulter). Secreted recombinant protein concentrations in cell culture supernatants were determined using an Octet® instrument (ForteBio) with IgG calibrators and protein A biosensors. Western blot analysis was undertaken on standard laboratory equipment. Microscopy images were collected on a Zeiss LSM 880/Elyra/Axio Observer Z1 confocal microscope. Mass spectrometry data was collected on a Synapt G2Si (Waters) mass spectrometer. The data was analyzed using the Waters software UNIFI searching a Waters Lipid Maps database.
Data format	Raw; this manuscript, reference [3] and http://doi.org/10.5281/zenodo.3610075. doi: 10.5281/zenodo.3610075 Analyzed
Parameters for data collection	Control, SCD1 and SREBF1 engineered Chinese hamster ovary cells were cultured under standard batch conditions and transfected to transiently express a range of model secreted biotherapeutic recombinant proteins.
Description of data collection	Western blot and densitometry analysis was undertaken to investigate SCD1 and SREBF1 expression. Immunofluorescence analysis was undertaken to analyze expression and cellular localization of SCD1 and SREBF1. Cell counting was undertaking to determine viable cell numbers and culture viability during batch culture. An Octet® instrument and western blotting was used to estimate secreted recombinant protein yields. Mass spectrometry was undertaken to investigate specific lipids in control, SCD1 and SREBF1 engineered cells.
Data source location	Institution: University of Kent City/Town/Region: Canterbury Country: UK
Data accessibility	With the article, and/or in Refs. [1,3], and in the Zendo data repository; https://doi.org/10.5281/zenodo.3610075.
Related research article	Budge JD, Knight TJ, Povey J, Roobol J, Brown IR, Singh G, Dean A, Turner S, Jaques CM, Young RJ, Racher AJ, Smales CM (2020) Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production, Metabolic Engineering, 57:203–216. https://doi.org/10.1016/j.ymben.2019.11.007.