Figure 6.

KLF10 Inhibits the Migration and Invasion of BCa Cells and Is Regulated by miR-570-3p

(A) Schematic diagram showing two potential binding sites between miR-570-3p and the 3′ UTR of KLF10 and also showing the mutated bases in the 3′ UTR of KLF10 for the following luciferase reporter assay. (B) A luciferase reporter assay in BCa cells was applied to detect the interaction between miR570-3p mimic and psiCHECK-2-wild-type KLF10 (KLF10 3′ UTR-wt) or psiCHECk-2 mutant type KLF10 (KLF10 3′ UTR-mut) plasmids. (C and D) The migration and invasion abilities of T24 and UM-UC-3 cells were enhanced after silencing KLF10 assessed by Transwell and wound-healing assays. (E and F) Western blot assay indicated that miR-570-3p inhibited KLF10 expression and promoted the expression of Slug (E) and that knockdown of KLF10 by siRNAs could upregulate the expression of Slug (F). Scale bars, 100 μm. Data are presented as the mean ± SEM of three experiments. *p < 0.05; **p < 0.01, Student’s t test.