Figure 8.

The Effects of circFUT8 and miR-570-3p on LN Metastasis of BCa Cells In Vivo

(A) Representative image of the nude mouse model of popliteal LN metastasis. (B) The image of enucleated popliteal LNs. The volume of LNs was significantly decreased after circFUT8 treatment for 4 weeks. (C) Representative images of H&E staining of the popliteal LNs indicate that tumor-associated LN metastasis existed in the vector group but not the circFUT8 group. (D) IHC staining of KLF10 and Slug in tumor from footpads with vector and circFUT8 treatment. (E) The volume of LNs was significantly increased after miR-570-3p treatment for 3 weeks. (F) H&E staining of the popliteal LNs indicates that miR-570-3p promoted the LN metastasis of UM-UC-3 cells. (G) IHC staining of KLF10 and Slug in tumor from footpads with vector and miR-570-3p treatment. (H) Schematic diagram indicates that circFUT8 inhibited the metastasis of BCa cells through the miR-570-3p/KLF10/Slug axis. Scale bars, 100 μm. Data are presented as the mean ± SEM of three experiments. *p < 0.05; **p < 0.01, Student’s t test.