Figure 4.

Single-Cell Sequencing Demonstrates that hMLL1 Induction Influences the Heterogeneity of the c-Kit⁺/Cd41⁺ Population

(A) Unsupervised hierarchical clustering of gene expression for filtered cells, ordered by average log fold change (see Supplemental Experimental Procedures and Table S1). The top 15 (clusters 1 and 2) or top 37 (cluster 3) genes enriched in each cluster are shown in a heatmap with gene expression represented on a log scale from red to blue (high to low).

(B) T-distributed stochastic neighbor embedding (t-SNE) plot of WT (KH2) and hMLL1-induced c-Kit⁺/Cd41⁺ cells colored by clusters according to (A).

(C) Proportion of cells within each cluster is shown in the bar graph.

(D) WT or hMLL1-induced cells are arranged by pseudotime axis (see Supplemental Experimental Procedures) with cells colored by clusters according to (A).