Figure 5.

Genetic Programs and Molecular Pathways Associated with hMLL1 Induction

(A) IPA analysis to show the top pathways (by p value) regulated by hMLL1 induction.

(B) Venn diagram displays overlap of the top three major signaling pathways by p value affected by hMLL1 induction. Expression heatmap of overlap genes is shown on the right.

(C) Quantitative RT-PCR validates the upregulation of Rac/Rho/integrin signaling in hMLL1-induced c-Kit⁺/Cd41⁺ cells. Data show expression relative to Gapdh determined by qRT-PCR from three independently sorted samples ± SEM.

(D) Representative images showing F-actin visualized with rhodamine-conjugated phalloidin in Cd41⁺ enriched cells ± hMLL1 induction. Arrows indicate spreading actin stress fibers. At least 80 cells in each sample were quantified, and cell areas were measured using ImageJ software. Data represent the average ± SEM from two independent experiments.