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. 2020 Jan 15;12(1):292–307.

Figure 3.

Figure 3

Gene expression over 7 and 14 days of hyperoxia induced lung damage in C57 BL/6 mice, Hyperoxic vs. Normoxic lung samples. A. Nfkb1, Il-6 and Tgfβ1 gene expression 7 days post cell treatment. There was a statistically significant difference between lungs treated with iPSC vs. diPSC in regards to Tgfβ1 expression, P<0.01. There was also a statistically significant difference in Il-6 expression between the iPSC treated group and the normoxia and diPSC-treated groups P<0.05. B. Nfkb1, Il-6 and Tgfβ1 gene expression 14 days post cell treatment. Il-6 expression, there was a significant difference between the hyperoxia alone group and all others P<0.01-P<0.001. Tgfβ1 expression, there was a significant difference between normoxia and both the hyperoxia P<0.05 and diPSC-treated groups P<0.001, the hyperoxia and HBSS-treated group, P<0.01, and finally between the diPSC treated group and both the HBSS group, P<0.001 and iPSC group, P<0.05. Lastly, Nfkb1 was significantly increased in the hyperoxia alone and diPSC treated groups compared with the normoxia control, P<0.001 and also compared with the HBSS and iPSC treated groups P<0.001. °P<0.05, °°P<0.01, °°°P<0.001 between the indicated group and the normoxia control, *P<0.05, **P<0.01, ***P<0.001 between the indicated groups. C. Nfkb1, Il-6 and Tgfβ1 gene expression 7 days post cell treatment. There was a statistically significant difference between hyperoxia alone and lungs treated with iPSCs, diPSCs and HBSS in regards to Nfkb1, Il-6 and Tgfβ1 expression, P<0.001. All groups were also significantly different from normoxia, with the exception of hyperoxia, P<0.001. D. Nfkb1, Il-6 and Tgfβ1 gene expression 14 days post cell treatment. Il-6 expression, there was a significant difference between diPSC-treated lungs vs. iPSCs, P<0.001 and diPSC-treated lungs vs. HBSS, P<0.05. Differences were also seen in iPSC-treated lungs vs. normoxia, P<0.01 and diPSC-treated vs. normoxia, P<0.05. Tgfβ1 expression, there was a significant difference between normoxia and both the HBSS P<0.05 and iPSC-treated groups P<0.001. Expression was increased in iPSCs vs. HBSS, hyperoxia and diPSC-treated lungs, P<0.001. There were significant differences between diPSCs vs. hyperoxia, (P<0.01) and HBSS (P<0.001) treated groups. Lastly, Nfkb1 was significantly increased in the iPSC-treated lungs vs. the normoxic group (P<0.001) and diPSC-treated lungs (P<0.001). There was also a significant difference in the diPSC-treated group vs. HBSS and hyperoxia, P<0.001. °P<0.05, °°P<0.01, °°°P<0.001 between the indicated group and the normoxia control, *P<0.05, **P<0.01, ***P<0.001 between the indicated groups.