Figure 1.

F1/F3 gel inhibits the proliferation of TC-1 and Hela cells. (A-D) Approximately 5×10³ of TC-1 cells (A and B) or HeLa cells (C and D) were cultured either in media alone; with different concentrations (5 and 15 µg/ml) of F1 gel, F3 gel, F1/F3 gel, or P3 gel; with 15 µg/ml of F1, F3, or P3 peptides in PBS; or with gel only overnight before MTT assay was performed. Each bar represents the means of three replicates, and the error bars represent the standard deviations. The data are representative of two independent experiments. *P<0.05; **P<0.01; ****P<0.0001; ns, not significant. (E-H) IC₅₀ values for F1, F3, and F1/F3 in free peptide in PBS or gel form were determined in cultures of 5×10³ TC-1 cells (E and G) and HeLa cells (F and H). Cells were cultured in media alone or with different concentrations (1.5625, 3.125, 6.25, 12.5, 25, and 50 µg/ml) of the F1 gel, F3 gel, F1/F3 gel, P3 gel, F1 peptide, F3 peptide, or P3 peptide in PBS overnight before the MTT assay was performed. Each bar represents the means of three replicates, which were performed in triplicate, and the error bars represent the standard deviations. The result is the representative of three independent experiments.