Figure 5.

Topically applied F1/F3 gel promotes infiltration of T cells and NK cells to the TC-1 tumor site. C57/BL6 mice were subcutaneously transplanted with 5×10⁵ TC-1 tumor cells. After 3 days, P3 gel or F1/F3 gel (n=8/group) was applied daily to the shaved skin area above the tumor for 7 days consecutively. Mice were sacrificed 2 days after the final treatment, and (A) isolated tumors were weighed using an electronic balance. Five tumors from each group were randomly selected for analysis. Single cell suspensions were stained with anti-CD45.2, anti-CD3, and anti-NK1.1 antibodies and subjected to flow cytometry. (B) Infiltration of CD45+ cells in tumor tissues. (C) Infiltration of CD45+CD3+ T cells in tumor tissues. (D) Infiltration of CD45+NK1.1+ cells in tumor tissues. PI was used to exclude dead cells. One representative result from three identical experiments is shown. Data are shown for each individual mouse, and means and standard deviations are indicated by bars. *P<0.05; **P<0.01; ns, not significant.