Figure 4.
Characterization of a Sox100B Activity Reporter
(A) Schematic diagram showing the generation of Sox21a1st intron dsRed reporter line. The entire first intron of Sox21a (shown in green) was cloned into dsRed reporter construct to drive dsRed expression in vivo.
(B and C) Representative confocal images of posterior midgut showing that Sox21a1st intron dsRed expression overlaps with esgGal4-driven GFP expression. Zoom in images of the outlined region is shown in (C).
(D) Representative confocal images of posterior midguts illustrating that Sox21a1st intron dsRed reporter expression is almost abolished in 5 day Sox100BRNAi-expressing ISC/EBs. Delta and Prospero staining are separated by cellular localization (Delta, membrane staining; Prospero, nuclear staining).
(E) Quantification of a blind scoring experiment based on dsRed intensity. The number of guts scored for each condition is indicated above the bar, and p values are calculated using Fisher's exact test (p < 10−5 versus control). ∗∗∗∗p < 0.0001.
(F) Representative confocal images of L3 stage larval testes showing that Sox21a1st intron dsRed expression completely overlaps with Sox100B protein expression and is Sox100B dependent in the testis pigment cells. The expression of Sox100B and Sox21a1st intron dsRed are both absent in Sox100Bd1 homozygous null mutant testes.