Figure 3.

Flow Cytometry Assessment of M1/M2-Like Markers on Infiltrating Monocyte-Macrophages. Isolated cells obtained from lungs at 7 days post-administration of TDM were examined by flow cytometry for expression of M1-like markers CD38 and CD86 (top), and for M2-like markers CD206 and EGR-2 (bottom). Isolated cells were first gated to identify CD11b^hiCD45^hi macrophages, then further analyzed for surface expression; Representative histographic plots of data accompany dot plots, side scatter (SSC) is shown versus signal intensity, for TDM-treated (blue) or wild type (WT) controls (orange). Analysis on the right indicates average (Avg) percent values with SD for each marker indicated. n = 4 to 6 mice.***P < 0.001 versus control and SD.