Location and abundance of COL6 in the mouse lung. A: Representative images of airway, alveolar, and blood vessel regions from wild-type (WT) and Col6a1−/− mice. Insets: IgG controls. B: Representative Western blot analysis of protein lysates from WT, Col6a1+/−, and Col6a1−/− lungs. C: Quantification of band intensity from COL6 Western blot analysis relative to intensity of β-actin, expressed as the proportion to WT lungs. n = 6 per group (B); n = 3 per group (C). ***P < 0.001 versus WT. Scale bars = 25 μm (A).