Figure 3.
Biomarker expression is similar in all groups. A: Cervical sections were stained for p16Ink4a (green). Nuclei were stained with Hoechst 33258 and pseudocolored red. Nontransgenic mouse cervix was used as a negative control. B: Cervical sections were stained for estrogen receptor (ER)-α (green). Nuclei were stained with Hoechst 33258 and pseudocolored red. Cervical sections from Esr1 knockout mice (ERαKO) were used as a negative control. C: Cervical sections were stained for progesterone receptor (PR) (green). Nuclei were stained with Hoechst 33258 and pseudocolored red. Cervical sections from Pgr knockout mice (PRKO) were used as a negative control. Dashed lines demarcate the border between the epithelium (ep) and stroma (st). Scale bars = 50 μm.