Table 1.

Antibodies and Conditions for IHC

Antibody	Manufacturer	Cat no.	Clone no.	Blocking buffer	Antibody dilution
BrdU	EMD Biosciences, San Diego, CA	NA61	Mobu-1	5% goat serum	1:50
ERα	Immunotech, Marseille, France	IM1545	1D5	5% goat serum	1:100
Ki-67	Thermo Scientific, Fremont, CA	RM-9106	SP6	5% goat serum	1:100
MCM7∗	Lab Vision, Fremont, CA	MS-862	47DC141	0.5% skim milk + 5% goat serum	1:200
PR	Sigma-Aldrich, St. Louis, MO	SAB5500165	SP2	5% goat serum	1:1000
p16Ink4a	Rockland Immunochemicals, Limerick, PA	600-401-AJ9	Polyclonal	5% goat serum	1:200

BrdU, bromodeoxyuridine; ER, estrogen receptor; MCM, minichromosome maintenance complex component; PR, progesterone receptor.

^∗Discontinued.