Figure 4.

Effect of PMQ on the protein levels of sestrins, Keap1, and Nrf2 downstream targets HO-1 in the cardiac tissue of MSG-IO mice. (a) Representative immunoblot and relative density analysis of sestrin1; (b) representative immunoblot and relative density analysis of sestrin2; (c) representative immunoblot and relative density analysis of Keap1; and (d) representative immunoblot and relative density analysis of HO-1. The relative density was expressed as the ratio (sestrin1/2, Keap1, and HO-1/GAPDH). Data are expressed as mean ± SEM, n = 6. ^∗∗∗P < 0.001 vs. control group; ^ΔP < 0.05, ^ΔΔP < 0.01 vs. MSG group.