Table 1.
Chromatographic identification of the main bioactive components found in affron® by HPLC coupled to the mass detector in ESI+ and ESI−.
| RT (minutes) | Compound | Maximum UV-Vis (nm) | ESI+ (m/z) | ESI− (m/z) |
|---|---|---|---|---|
| 7.6 | Picrocrocin | 251 | 511.2; 365.1; 353.2; 337.1; 329.1; 185.1; 159.9; 151.1; 123.0; 81.3 | 1480.8; 667.2; 517.1; 385.0; 181.2; 153.1 |
| 11.5 | Kaempferol diglucoside | 266; 347 | 633.2; 347.1 | 609.0; 284.0 |
| 22.1 | trans-crocin-4 | 261; 323; 441; 464 | 999.4; 675.2; 511.2; 347.1 | 1336.2; 651.2; 326.8; 283.1 |
| 26.7 | trans-crocin-3 | 261; 323; 440; 463 | 837.4 | |
| 30.7 | trans-crocin-2′ | 261; 328; 440; 464 | 675.2; 593.0; 513.2; 351.1; 130.1; 102.2; 74.1 | |
| 33.9 | Safranal | 313 | — | — |
| 36.2 | cis-crocin-4 | 225; 262; 325; 433; 456 | 999.4; 821.3; 668.3; 611.0; 391.0; 347.0 | 651.2 |
| 37.6 | trans-crocin-2 | 260; 322; 433; 458 | 675.2; 541.1 | 651.2; 327.1; 311.1; 283.1 |
Bold numbers indicate coincidences in the molecular fractions obtained in [30].