Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used in this study.
| Target gene | Primer direction | Nucleotide sequence (5′–3′) | Amplicon size (bp) | Reference number |
|---|---|---|---|---|
| intI1 | F | CAGTGGACATAAGCCTGTTC | 160 | 15 |
| R | CCCGAGGCATAGACTGTA | |||
| sul1 | F | CGGCGTGGGCTACCTGAACG | 433 | 15 |
| R | GCCGATCGCGTGAAGTTCCG | |||
| qacEΔ1 | F | ATCGCAATAGTTGGCGAAGT | 250 | 15 |
| R | GAAGCTTTTGCCCATGAAGC | |||
| Class 1 gene cassette | F | GGCATCCAAGCAGCAAGC | Variable | 15 |
| R | AAGCAGACTTGACCTGAT | |||
| aggR | F | GTATACACAAAAGAAGGAAGC | 254 | 10 |
| R | ACAGAATCGTCAGCATCAGC | |||
| eae | F | GCTTAGTGCTGGTTTAGGAT | 591 | 10 |
| R | CTCTGCAGATTAACCTCTGC |
