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. 2020 Jan 1;13(1):1–13.

Figure 5.

Figure 5

ADAM19 was a direct target of miR-30a-5p. A. The binding sites between miR-30a-5p and ADAM19 were provided by TargetScan. B. The luciferase activity was measured in A549 and SCLC-21H cells co-transfected with WT-ADAM19 or MUT-ADAM19 and miR-30a-5p or miR-NC. C. The expression of ADAM19 was measured using qRT-PCR in lung cancer tissues and adjacent normal samples. D. The correlation between ADAM19 mRNA level and miR-30a-5p expression was analyzed in lung cancer tissues using Spearman’s correlation analysis. E. The protein expression of ADAM19 was examined by western blot in in lung cancer tissues and adjacent normal tissues. F and G. The mRNA and protein levels of ADAM19 were detected in cells (HBE, A549 and SCLC-21H) by qRT-PCR and western blot, respectively. H. The abundance of miR-30a-5p was measured by qRT-PCR in A549 and SCLC-21H cells transfected with anti-miR-NC or anti-miR-30a-5p. I and J. The mRNA and protein expression levels of ADAM19 were detected in A549 and SCLC-21H transfected with miR-NC, miR-30a-5p, anti-miR-NC, or anti-miR-30a-5p by qRT-PCR and western blot, respectively. *P<0.05.