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. 2020 Jan 18;21(2):640. doi: 10.3390/ijms21020640

Figure 2.

Figure 2

The cytokinin contents were increased in JcTM6:AtIPT4 transgenic J. curcas. (A) Relative expression of AtIPT4, JcAHK2, and JcARR3 in the inflorescence buds of the wild-type (WT) and transgenic J. curcas plants. The levels were normalized using the amplified products of JcAct1. The values are presented as the means ± standard deviations (n = 3). (B) Six cytokinin species in the inflorescence buds of transgenic and WT plants were examined. iP, isopentenyladenine; iPR, isopentenyladenosine; tZ, trans-zeatin; tZR, trans-zeatin riboside; cZ, cis-zeatin; and cZR, cis-zeatin riboside. The values represent the means ± standard deviations (n = 3). Student’s t-test was used for the statistical analyses: * p ≤ 0.05, ** p ≤ 0.01.