Skip to main content
. 2020 Jan 15;21(2):557. doi: 10.3390/ijms21020557

Figure 1.

Figure 1

Indomethacin induced endoplasmic reticulum (ER) stress in H4 cells. H4 cells were treated with indomethacin (0 and 250 µM) over time (A). H4 cells were treated with various concentrations of indomethacin (0–500 µM) for 5 h (B). Proteins were isolated and subjected to Western blot with indicated antibodies. Representative blot of three independent experiments is shown. H4 cells were treated with indomethacin (0 and 250 µM) in the presence of salubrinal (0 and 5 µM). Cell viability (24 h) was assessed by MTS reduction assay (C). Caspase 3 activity (5 h) was assessed by enzymatic assay (D). * p < 0.05 vs. untreated control and # p < 0.05 vs. indomethacin alone control (250 µM), n = 3.