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. 2020 Jan 13;21(2):498. doi: 10.3390/ijms21020498

Figure 3.

Figure 3

Detection of PIV5 antigen and RNA and their association to sites of inflammation. A, B: Histopathological analysis. Hematoxylin and eosin (H&E) staining of the formatio reticularis of animal 26731. Signs of non-suppurative encephalitis are present as perivascular cuffs (A, arrowheads) and glial nodes (B, arrows). C, D: Detection of viral antigen by immunohistochemistry (IHC) using the antibody PIV5 V5 TAG clone 2F11F7. In the formatio reticularis of animal 26731 (C), PIV5 antigen (red) can be seen in neurons, whereas in negative control animal 31292 (D), no PIV5 antigen can be detected. E, F: Detection of viral RNA by in situ hybridization (ISH) using the PIV5 RNAscope probe. In the formatio reticularis of animal 26731 (E), PIV5 RNA (arrows, brown) can be seen in neurons, whereas in negative control animal 31292 (F), no PIV5 RNA can be detected. (G,H) Detection of viral RNA with ISH using the PIV5 RNAscope probe. In the rhinal cortex of animal 26731 (G), PIV5 RNA (brown) shows no clear association to a specific cell type. In negative control animal 31292 (H), no PIV5 RNA can be detected. The scale bars at the bottom of the microphotographs correspond to 20 µm (A,B,G), and 10 µm (C–F,H), respectively.