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. 2020 Jan 13;21(2):498. doi: 10.3390/ijms21020498

Figure 5.

Figure 5

Correlation of BoAstV CH13 RNA to sites of inflammation. (A,B) Histopathological analysis. Hematoxylin and eosin (H&E) staining of the midbrain of animal 26875. Signs of non-suppurative encephalitis are present as perivascular cuffs (A, arrowheads) and glial nodes (B, arrows). (C,D) Detection of viral RNA with in situ hybridization (ISH) using the BoAstV RNAscope probe. In the midbrain of animal 26875 (C), BoAstV CH13 RNA (brown) can be seen in neurons, whereas in negative control animal 44285 (D), no BoAstV CH13 RNA can be detected. Scale bars: 20 µm (A,B) and 10 µm (C,D).