Table 1.
Sequence of the oligonucleotides used for PCR.
| Segment | Sequences (5′-3′) | Positions (Amino Acids) | |
|---|---|---|---|
| N1 | N1-F | CGGGATCC ATGGCCGCACCAGTAGTC | 1-124 |
| N1-R | CCCTCGAG TAGCAGCTGATGTTTAGGATT | ||
| N2 | N2-F | CGGGATCC TCGGGAGCTGACACTTCTATTA | 113-240 |
| N2-R | CCCTCGAG TGCCCCTGCCTGAAAGTTG | ||
| N3 | N3-F | CGGGATCC AAGACGGGTATGGCTGATCC | 221-342 |
| N3-R | CCCTCGAG CTACGCTGCTGATTCCTGCT | ||
| N3-1 | N3-1-F | CGGGATCC TCTCGTACTGGTGCCAATGTCG | 221-267 |
| N3-1-R | CCCTCGAG GAGCGCATCCTTAAGTCTCTCATAG | ||
| N3-2 | N3-2-F | CGGGATCC TTCTCTTACTCAATCACAGTCAAGG | 261-307 |
| N3-2-R | CCCTCGAG GACTGGTCTTGTTTGTCAGGCTT | ||
| N3-3 | N3-3-F | CGGGATCC CCTGACAAACAAGACCAGTCTG | 301-342 |
| N3-3-R | CCCTCGAG CGCTGCTGATTCCTGCTTTA | ||
| N3-3-1 | N3-3-1-F | CGGGATCC CCTGACAAACAAGACCAGTCTGCTA | 301-330 |
| N3-3-1-R | CCCTCGAG CCACTCCCAATCCTGTTTGTCTG | ||
| N3-3-2 | N3-3-2-F | CGGGATCC GCTAAACCCAAACAGCAGAAGAAAC | 308-342 |
| N3-3-2-R | CCCTCGAG CGCTGCTGATTCCTGCTTTAT | ||
| N3-3-3 | N3-3-3-F | CGGGATCC AAGACGGGTATGGCTGATCC | 221-328 |
| N3-3-3-R | CCCTCGAG CCAATCCTGTTTGTCTGCTG | ||
| N3-3-4 | N3-3-4-F | CGGGATCC AAGACGGGTATGGCTGATCC | 221-318 |
| N3-3-4-R | CCCTCGAG CTTTTTAGGTTTCTTCTGCTGTTTG |
Restriction endonuclease sites: BamHI (underlined) and XhoI (italic).